negative control plasmids Search Results


jaime modiano  (Addgene inc)
90
Addgene inc jaime modiano
Jaime Modiano, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/negative+control+plasmids/beta4-nAChR+(DPM+negative+control)+(Plasmid+%2386651)/10__1016_slash_j__jbc__2021__100402-223-6-8
Average 90 stars, based on 1 article reviews
jaime modiano - by Bioz Stars, 2026-10
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pten mock plasmids  (Addgene inc)
90
Addgene inc pten mock plasmids
Fig. 6. Role of Akt in Nrf2 activation. (A) Cells were treated with cis-GS (25 lM) for the indicted intervals and then collected for western blot analysis of pAkt and Nrf2 levels. (B) Cells were treated with 25 lM of LY294002, U0126, SP600125 or SB203580 for 1 h, prior to incubation with cis-GS (25 lM) for additional 6 h. HO-1 expression was determined by western blot analysis. (C) Cells were pretreated with LY294002 (25 lM) for 1 h and then treated with cis-GS for another 1 h and then nuclear levels of Nrf2 were analyzed by western blot analysis. (D) Cells were treated with cis-GS (25 lM) for 1 h after preincubation with or without NAC (5 mM) or DTT (0.5 mM) for 1 h. Levels of pAkt were analyzed by western blot analysis. (E) MCF10A cells were transfected with different <t>PTEN</t> <t>plasmids</t> as described in Materials and methods. Cells were treated with cis-GS (25 lM) for 6 h and then harvested for western blot analysis of PTEN, pAkt, Nrf2 and HO-1 protein levels. HA levels were included to ensure the transfection efficiency.
Pten Mock Plasmids, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/negative+control+plasmids/PTEN-mut+(negative+control)+(Plasmid+%2386646)/pm22095074-53-14-20
Average 90 stars, based on 1 article reviews
pten mock plasmids - by Bioz Stars, 2026-10
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negative control plasmid  (Addgene inc)
92
Addgene inc negative control plasmid
Fig. 6. Role of Akt in Nrf2 activation. (A) Cells were treated with cis-GS (25 lM) for the indicted intervals and then collected for western blot analysis of pAkt and Nrf2 levels. (B) Cells were treated with 25 lM of LY294002, U0126, SP600125 or SB203580 for 1 h, prior to incubation with cis-GS (25 lM) for additional 6 h. HO-1 expression was determined by western blot analysis. (C) Cells were pretreated with LY294002 (25 lM) for 1 h and then treated with cis-GS for another 1 h and then nuclear levels of Nrf2 were analyzed by western blot analysis. (D) Cells were treated with cis-GS (25 lM) for 1 h after preincubation with or without NAC (5 mM) or DTT (0.5 mM) for 1 h. Levels of pAkt were analyzed by western blot analysis. (E) MCF10A cells were transfected with different <t>PTEN</t> <t>plasmids</t> as described in Materials and methods. Cells were treated with cis-GS (25 lM) for 6 h and then harvested for western blot analysis of PTEN, pAkt, Nrf2 and HO-1 protein levels. HA levels were included to ensure the transfection efficiency.
Negative Control Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/negative+control+plasmids/alpha7-nAChR+(DPM+negative+control)+(Plasmid+%2386648)/pm36684652-47-8-18
Average 92 stars, based on 1 article reviews
negative control plasmid - by Bioz Stars, 2026-10
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negative control plasmid  (VectorBuilder GmbH)
86
VectorBuilder GmbH negative control plasmid
Fig. 6. Role of Akt in Nrf2 activation. (A) Cells were treated with cis-GS (25 lM) for the indicted intervals and then collected for western blot analysis of pAkt and Nrf2 levels. (B) Cells were treated with 25 lM of LY294002, U0126, SP600125 or SB203580 for 1 h, prior to incubation with cis-GS (25 lM) for additional 6 h. HO-1 expression was determined by western blot analysis. (C) Cells were pretreated with LY294002 (25 lM) for 1 h and then treated with cis-GS for another 1 h and then nuclear levels of Nrf2 were analyzed by western blot analysis. (D) Cells were treated with cis-GS (25 lM) for 1 h after preincubation with or without NAC (5 mM) or DTT (0.5 mM) for 1 h. Levels of pAkt were analyzed by western blot analysis. (E) MCF10A cells were transfected with different <t>PTEN</t> <t>plasmids</t> as described in Materials and methods. Cells were treated with cis-GS (25 lM) for 6 h and then harvested for western blot analysis of PTEN, pAkt, Nrf2 and HO-1 protein levels. HA levels were included to ensure the transfection efficiency.
Negative Control Plasmid, supplied by VectorBuilder GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/negative+control+plasmids/negative+control+plasmid/pmc11694445-90-4-12
Average 86 stars, based on 1 article reviews
negative control plasmid - by Bioz Stars, 2026-10
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negative control plasmids plvx-ires-zsgreen  (Shanghai GenePharma)
90
Shanghai GenePharma negative control plasmids plvx-ires-zsgreen
Fig. 6. Role of Akt in Nrf2 activation. (A) Cells were treated with cis-GS (25 lM) for the indicted intervals and then collected for western blot analysis of pAkt and Nrf2 levels. (B) Cells were treated with 25 lM of LY294002, U0126, SP600125 or SB203580 for 1 h, prior to incubation with cis-GS (25 lM) for additional 6 h. HO-1 expression was determined by western blot analysis. (C) Cells were pretreated with LY294002 (25 lM) for 1 h and then treated with cis-GS for another 1 h and then nuclear levels of Nrf2 were analyzed by western blot analysis. (D) Cells were treated with cis-GS (25 lM) for 1 h after preincubation with or without NAC (5 mM) or DTT (0.5 mM) for 1 h. Levels of pAkt were analyzed by western blot analysis. (E) MCF10A cells were transfected with different <t>PTEN</t> <t>plasmids</t> as described in Materials and methods. Cells were treated with cis-GS (25 lM) for 6 h and then harvested for western blot analysis of PTEN, pAkt, Nrf2 and HO-1 protein levels. HA levels were included to ensure the transfection efficiency.
Negative Control Plasmids Plvx Ires Zsgreen, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/negative+control+plasmids/negative+control+plasmids+plvx+ires+zsgreen/pmc06645689-29-4-10
Average 90 stars, based on 1 article reviews
negative control plasmids plvx-ires-zsgreen - by Bioz Stars, 2026-10
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negative control plasmid (pc-nc)  (Shanghai GenePharma)
90
Shanghai GenePharma negative control plasmid (pc-nc)
Fig. 6. Role of Akt in Nrf2 activation. (A) Cells were treated with cis-GS (25 lM) for the indicted intervals and then collected for western blot analysis of pAkt and Nrf2 levels. (B) Cells were treated with 25 lM of LY294002, U0126, SP600125 or SB203580 for 1 h, prior to incubation with cis-GS (25 lM) for additional 6 h. HO-1 expression was determined by western blot analysis. (C) Cells were pretreated with LY294002 (25 lM) for 1 h and then treated with cis-GS for another 1 h and then nuclear levels of Nrf2 were analyzed by western blot analysis. (D) Cells were treated with cis-GS (25 lM) for 1 h after preincubation with or without NAC (5 mM) or DTT (0.5 mM) for 1 h. Levels of pAkt were analyzed by western blot analysis. (E) MCF10A cells were transfected with different <t>PTEN</t> <t>plasmids</t> as described in Materials and methods. Cells were treated with cis-GS (25 lM) for 6 h and then harvested for western blot analysis of PTEN, pAkt, Nrf2 and HO-1 protein levels. HA levels were included to ensure the transfection efficiency.
Negative Control Plasmid (Pc Nc), supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/negative+control+plasmids/negative+control+plasmid/pm31881486-69-7-14
Average 90 stars, based on 1 article reviews
negative control plasmid (pc-nc) - by Bioz Stars, 2026-10
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negative control oe-nc  (Shanghai GenePharma)
90
Shanghai GenePharma negative control oe-nc
Fig. 6. Role of Akt in Nrf2 activation. (A) Cells were treated with cis-GS (25 lM) for the indicted intervals and then collected for western blot analysis of pAkt and Nrf2 levels. (B) Cells were treated with 25 lM of LY294002, U0126, SP600125 or SB203580 for 1 h, prior to incubation with cis-GS (25 lM) for additional 6 h. HO-1 expression was determined by western blot analysis. (C) Cells were pretreated with LY294002 (25 lM) for 1 h and then treated with cis-GS for another 1 h and then nuclear levels of Nrf2 were analyzed by western blot analysis. (D) Cells were treated with cis-GS (25 lM) for 1 h after preincubation with or without NAC (5 mM) or DTT (0.5 mM) for 1 h. Levels of pAkt were analyzed by western blot analysis. (E) MCF10A cells were transfected with different <t>PTEN</t> <t>plasmids</t> as described in Materials and methods. Cells were treated with cis-GS (25 lM) for 6 h and then harvested for western blot analysis of PTEN, pAkt, Nrf2 and HO-1 protein levels. HA levels were included to ensure the transfection efficiency.
Negative Control Oe Nc, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/negative+control+plasmids/negative+control+plasmid+sh+nc/pm35761814-44-9-44
Average 90 stars, based on 1 article reviews
negative control oe-nc - by Bioz Stars, 2026-10
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negative control (nc) plasmids  (Shanghai GenePharma)
90
Shanghai GenePharma negative control (nc) plasmids
Fig. 6. Role of Akt in Nrf2 activation. (A) Cells were treated with cis-GS (25 lM) for the indicted intervals and then collected for western blot analysis of pAkt and Nrf2 levels. (B) Cells were treated with 25 lM of LY294002, U0126, SP600125 or SB203580 for 1 h, prior to incubation with cis-GS (25 lM) for additional 6 h. HO-1 expression was determined by western blot analysis. (C) Cells were pretreated with LY294002 (25 lM) for 1 h and then treated with cis-GS for another 1 h and then nuclear levels of Nrf2 were analyzed by western blot analysis. (D) Cells were treated with cis-GS (25 lM) for 1 h after preincubation with or without NAC (5 mM) or DTT (0.5 mM) for 1 h. Levels of pAkt were analyzed by western blot analysis. (E) MCF10A cells were transfected with different <t>PTEN</t> <t>plasmids</t> as described in Materials and methods. Cells were treated with cis-GS (25 lM) for 6 h and then harvested for western blot analysis of PTEN, pAkt, Nrf2 and HO-1 protein levels. HA levels were included to ensure the transfection efficiency.
Negative Control (Nc) Plasmids, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/negative+control+plasmids/negative+control++nc++plasmids/pm33109774-135-17-23
Average 90 stars, based on 1 article reviews
negative control (nc) plasmids - by Bioz Stars, 2026-10
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negative control empty pgcmv/egfp plasmid  (Shanghai GenePharma)
90
Shanghai GenePharma negative control empty pgcmv/egfp plasmid
Fig. 6. Role of Akt in Nrf2 activation. (A) Cells were treated with cis-GS (25 lM) for the indicted intervals and then collected for western blot analysis of pAkt and Nrf2 levels. (B) Cells were treated with 25 lM of LY294002, U0126, SP600125 or SB203580 for 1 h, prior to incubation with cis-GS (25 lM) for additional 6 h. HO-1 expression was determined by western blot analysis. (C) Cells were pretreated with LY294002 (25 lM) for 1 h and then treated with cis-GS for another 1 h and then nuclear levels of Nrf2 were analyzed by western blot analysis. (D) Cells were treated with cis-GS (25 lM) for 1 h after preincubation with or without NAC (5 mM) or DTT (0.5 mM) for 1 h. Levels of pAkt were analyzed by western blot analysis. (E) MCF10A cells were transfected with different <t>PTEN</t> <t>plasmids</t> as described in Materials and methods. Cells were treated with cis-GS (25 lM) for 6 h and then harvested for western blot analysis of PTEN, pAkt, Nrf2 and HO-1 protein levels. HA levels were included to ensure the transfection efficiency.
Negative Control Empty Pgcmv/Egfp Plasmid, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/negative+control+plasmids/negative+control+empty+pgcmv+egfp+plasmid/pmc05928679-40-7-15
Average 90 stars, based on 1 article reviews
negative control empty pgcmv/egfp plasmid - by Bioz Stars, 2026-10
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sequences and plasmids for umuc-3 transfection si- negative control (nc)  (Shanghai GenePharma)
90
Shanghai GenePharma sequences and plasmids for umuc-3 transfection si- negative control (nc)
Fig. 6. Role of Akt in Nrf2 activation. (A) Cells were treated with cis-GS (25 lM) for the indicted intervals and then collected for western blot analysis of pAkt and Nrf2 levels. (B) Cells were treated with 25 lM of LY294002, U0126, SP600125 or SB203580 for 1 h, prior to incubation with cis-GS (25 lM) for additional 6 h. HO-1 expression was determined by western blot analysis. (C) Cells were pretreated with LY294002 (25 lM) for 1 h and then treated with cis-GS for another 1 h and then nuclear levels of Nrf2 were analyzed by western blot analysis. (D) Cells were treated with cis-GS (25 lM) for 1 h after preincubation with or without NAC (5 mM) or DTT (0.5 mM) for 1 h. Levels of pAkt were analyzed by western blot analysis. (E) MCF10A cells were transfected with different <t>PTEN</t> <t>plasmids</t> as described in Materials and methods. Cells were treated with cis-GS (25 lM) for 6 h and then harvested for western blot analysis of PTEN, pAkt, Nrf2 and HO-1 protein levels. HA levels were included to ensure the transfection efficiency.
Sequences And Plasmids For Umuc 3 Transfection Si Negative Control (Nc), supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/negative+control+plasmids/sequences+and+plasmids+for+umuc+3+transfection+si++negative+control++nc+/pmc06861677-219-3-24
Average 90 stars, based on 1 article reviews
sequences and plasmids for umuc-3 transfection si- negative control (nc) - by Bioz Stars, 2026-10
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plasmids containing mimic negative control  (Shanghai GenePharma)
90
Shanghai GenePharma plasmids containing mimic negative control
Fig. 6. Role of Akt in Nrf2 activation. (A) Cells were treated with cis-GS (25 lM) for the indicted intervals and then collected for western blot analysis of pAkt and Nrf2 levels. (B) Cells were treated with 25 lM of LY294002, U0126, SP600125 or SB203580 for 1 h, prior to incubation with cis-GS (25 lM) for additional 6 h. HO-1 expression was determined by western blot analysis. (C) Cells were pretreated with LY294002 (25 lM) for 1 h and then treated with cis-GS for another 1 h and then nuclear levels of Nrf2 were analyzed by western blot analysis. (D) Cells were treated with cis-GS (25 lM) for 1 h after preincubation with or without NAC (5 mM) or DTT (0.5 mM) for 1 h. Levels of pAkt were analyzed by western blot analysis. (E) MCF10A cells were transfected with different <t>PTEN</t> <t>plasmids</t> as described in Materials and methods. Cells were treated with cis-GS (25 lM) for 6 h and then harvested for western blot analysis of PTEN, pAkt, Nrf2 and HO-1 protein levels. HA levels were included to ensure the transfection efficiency.
Plasmids Containing Mimic Negative Control, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/negative+control+plasmids/plasmids+containing+inhibitor+negative+control/pmc04509132-79-17-27
Average 90 stars, based on 1 article reviews
plasmids containing mimic negative control - by Bioz Stars, 2026-10
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negative control plasmid phblv cmvie  (Shanghai GenePharma)
90
Shanghai GenePharma negative control plasmid phblv cmvie
Fig. 6. Role of Akt in Nrf2 activation. (A) Cells were treated with cis-GS (25 lM) for the indicted intervals and then collected for western blot analysis of pAkt and Nrf2 levels. (B) Cells were treated with 25 lM of LY294002, U0126, SP600125 or SB203580 for 1 h, prior to incubation with cis-GS (25 lM) for additional 6 h. HO-1 expression was determined by western blot analysis. (C) Cells were pretreated with LY294002 (25 lM) for 1 h and then treated with cis-GS for another 1 h and then nuclear levels of Nrf2 were analyzed by western blot analysis. (D) Cells were treated with cis-GS (25 lM) for 1 h after preincubation with or without NAC (5 mM) or DTT (0.5 mM) for 1 h. Levels of pAkt were analyzed by western blot analysis. (E) MCF10A cells were transfected with different <t>PTEN</t> <t>plasmids</t> as described in Materials and methods. Cells were treated with cis-GS (25 lM) for 6 h and then harvested for western blot analysis of PTEN, pAkt, Nrf2 and HO-1 protein levels. HA levels were included to ensure the transfection efficiency.
Negative Control Plasmid Phblv Cmvie, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/negative+control+plasmids/negative+control+plasmid+phblv+cmvie/pm37354639-85-14-21
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negative control plasmid phblv cmvie - by Bioz Stars, 2026-10
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Image Search Results


Fig. 6. Role of Akt in Nrf2 activation. (A) Cells were treated with cis-GS (25 lM) for the indicted intervals and then collected for western blot analysis of pAkt and Nrf2 levels. (B) Cells were treated with 25 lM of LY294002, U0126, SP600125 or SB203580 for 1 h, prior to incubation with cis-GS (25 lM) for additional 6 h. HO-1 expression was determined by western blot analysis. (C) Cells were pretreated with LY294002 (25 lM) for 1 h and then treated with cis-GS for another 1 h and then nuclear levels of Nrf2 were analyzed by western blot analysis. (D) Cells were treated with cis-GS (25 lM) for 1 h after preincubation with or without NAC (5 mM) or DTT (0.5 mM) for 1 h. Levels of pAkt were analyzed by western blot analysis. (E) MCF10A cells were transfected with different PTEN plasmids as described in Materials and methods. Cells were treated with cis-GS (25 lM) for 6 h and then harvested for western blot analysis of PTEN, pAkt, Nrf2 and HO-1 protein levels. HA levels were included to ensure the transfection efficiency.

Journal: Carcinogenesis

Article Title: Guggulsterone induces heme oxygenase-1 expression through activation of Nrf2 in human mammary epithelial cells: PTEN as a putative target.

doi: 10.1093/carcin/bgr259

Figure Lengend Snippet: Fig. 6. Role of Akt in Nrf2 activation. (A) Cells were treated with cis-GS (25 lM) for the indicted intervals and then collected for western blot analysis of pAkt and Nrf2 levels. (B) Cells were treated with 25 lM of LY294002, U0126, SP600125 or SB203580 for 1 h, prior to incubation with cis-GS (25 lM) for additional 6 h. HO-1 expression was determined by western blot analysis. (C) Cells were pretreated with LY294002 (25 lM) for 1 h and then treated with cis-GS for another 1 h and then nuclear levels of Nrf2 were analyzed by western blot analysis. (D) Cells were treated with cis-GS (25 lM) for 1 h after preincubation with or without NAC (5 mM) or DTT (0.5 mM) for 1 h. Levels of pAkt were analyzed by western blot analysis. (E) MCF10A cells were transfected with different PTEN plasmids as described in Materials and methods. Cells were treated with cis-GS (25 lM) for 6 h and then harvested for western blot analysis of PTEN, pAkt, Nrf2 and HO-1 protein levels. HA levels were included to ensure the transfection efficiency.

Article Snippet: The pSG5L HA PTEN expressing (wild type), PTEN with C124S mutation (mutant type) and PTEN mock plasmids were purchased from addgene (Addgene, South Korea).

Techniques: Activation Assay, Western Blot, Incubation, Expressing, Transfection